| Date | Home | Away | Result |
|---|---|---|---|
| 15/08/25 17:00 | FC Gütersloh | 1. FC Union Berlin | 0-5 |
| 15/08/25 17:00 | 1. FC Saarbrücken | 1. FC Magdeburg | 1-3 |
| 15/08/25 17:00 | SG Sonnenhof Großaspach | Bayer 04 Leverkusen | 0-4 |
| 15/08/25 19:45 | Arminia Bielefeld | Werder Bremen | 1-0 |
| 16/08/25 12:00 | FK Pirmasens | Hamburger SV | 1-2a.e.t |
| 16/08/25 12:00 | BFC Dynamo | VfL Bochum | 1-3a.e.t |
| 16/08/25 14:30 | SV Hemelingen | VfL Wolfsburg | 0-9 |
| 16/08/25 14:30 | Eintracht Norderstedt | FC St. Pauli | 2-3a.PSO |
| 16/08/25 14:30 | FV Illertissen | 1. FC Nürnberg | 9-8a.PSO |
| 16/08/25 14:30 | FC Hansa Rostock | 1899 Hoffenheim | 0-4 |
| 16/08/25 14:30 | SV Sandhausen | RB Leipzig | 2-4 |
| 16/08/25 14:30 | Bahlinger SC | 1. FC Heidenheim 1846 | 0-5 |
| 16/08/25 17:00 | Sportfreunde Lotte | SC Freiburg | 0-2 |
| 16/08/25 17:00 | VfB Lübeck | SV Darmstadt 98 | 1-2 |
| 16/08/25 17:00 | Energie Cottbus | Hannover 96 | 1-0 |
| 17/08/25 12:00 | FC Viktoria Köln | SC Paderborn 07 | 1-3 |
| 17/08/25 12:00 | FV Engers 07 | Eintracht Frankfurt | 0-5 |
| 17/08/25 14:30 | RSV Eintracht 1949 | 1. FC Kaiserslautern | 0-7 |
| 17/08/25 14:30 | SV Atlas Delmenhorst | Bor. Mönchengladbach | 2-3 |
| 17/08/25 14:30 | ZFC Meuselwitz | Karlsruher SC | 0-5 |
| 17/08/25 14:30 | Blau-Weiß Lohne | SpVgg Greuther Fürth | 0-2 |
| 17/08/25 14:30 | Jahn Regensburg | 1. FC Köln | 1-2 |
| 17/08/25 14:30 | 1. FC Lokomotive Leipzig | FC Schalke 04 | 0-1a.e.t |
| 17/08/25 17:00 | Hallescher FC | FC Augsburg | 0-2 |
| 17/08/25 17:00 | SSV Ulm 1846 | SV Elversberg | 0-1 |
| 17/08/25 17:00 | FC 08 Homburg | Holstein Kiel | 0-2 |
| 18/08/25 17:00 | Preußen Münster | Hertha BSC | 3-5a.PSO |
| 18/08/25 17:00 | Dynamo Dresden | FSV Mainz 05 | 0-1 |
| 18/08/25 17:00 | 1. FC Schweinfurt 05 | Fortuna Düsseldorf | 2-4 |
| 18/08/25 19:45 | Rot-Weiss Essen | Borussia Dortmund | 0-1 |
| 26/08/25 19:45 | Eintracht Braunschweig | VfB Stuttgart | 11-12a.PSO |
| 27/08/25 19:45 | SV Wehen Wiesbaden | FC Bayern Munich | 2-3 |